CD105 expression is downregulated on confluent mASCs.

<p>Total mASCs were plated at a low density (5000 cells/cm²) and grown to confluence during 7 days. Cells were harvested on day 1, 3, 4 and 7 and (A) CD105 expression was analyzed by flow cytometry and confluence was assessed using the ImageJ software and (B) total mRNA was purified, reverse transcribed and used for analyzing the expression levels of CD105 and MMP-14. (C) Total ASCs were seeded at 5000 cells/cm² in 6 well plates and 4 days later, GM6001 (25 µM) or vehicle (DMSO) were added to the cells. Cells were harvested after 72 hours and CD105 expression was analyzed by flow cytometry. Data is shown as mean (SEM) of at least 3 independent experiments.