Enriching cells with NBD-PG does not alter the voltage dependence of fusion induced by influenza HA.

<p>HAb2 cells were used as effector (Eff) cells and either HEK 293T (Tg1, first two sets of columns) or Cos7 (Tg2, last two sets of columns) cells were used as targets. Regardless of target cell type, HA-mediated fusion remained independent of voltage after incorporating NBD-PG into either the effector (first and third set of columns) or target (second and fourth set of columns) cells. Voltage was switched from negative (shaded bars) to positive (open bars) by the addition of 30 µM SQI-Pr.