Organization of the predicted genes encoding the 2-aminophenol central pathway in <i>B. xenovorans</i> LB400 and comparison of bacterial <i>amn</i> gene clusters.

<p>A, The <i>amn</i> genes encoding the 2-aminophenol central pathway located at the major chromosome (C1). Genes of the <i>amn</i> gene cluster neighborhood are represented with white arrows. The genes located upstream of the <i>amn</i> gene cluster encode the following proteins: <i>clcR</i>, LysR-type transcriptional regulator of the 3-chlorocatechol pathway; BxeA1132, pseudogene; BxeA1134, LysR-type transcriptional regulator; BxeA1135, XRE-type transcriptional regulator; BxeA1136 LysR-type transcriptional regulator; BxeA1137, MarR-type transcriptional regulator; BxeA1138, TetR-type transcriptional regulator; BxeA1139, hypothetical protein; BxeA1140, DoxX family protein; BxeA1141, TetR-type transcriptional regulator; BxeA1142, DoxX family protein. The genes located downstream of <i>amn</i> gene cluster encode the following proteins: BxeA1153, putative esterase; BxeA1154, TraG conjugal transfer coupling protein; BxeA1155, outer membrane protein from MFS multidrug efflux system; BxeA1156, inner membrane protein from MFS multidrug efflux system; BxeA1157, transmembrane protein from MFS multidrug efflux system. The sizes of the genes and the intergenic regions are on scale. B, Comparison of bacterial <i>amn</i> gene clusters. The <i>amn</i> sequences (accession number) are: <i>B. xenovorans</i> LB400 (NC007951), <i>P. knackmussii</i> B13 (AJ617740), <i>Bordetella avium</i> 197N (NC010645), <i>P. putida</i> HS12 plasmid pNB1 (AF319593) and plasmid pNB2 (AF319592), <i>P. putida</i> W619 (CP000949), <i>Pseudomonas</i> sp. AP3 (AB020521), <i>P. pseudoalcaligenes</i> JS45 (PPU93363), <i>Comamonas testosteroni</i> CNB-1 plasmid pCNB1 (EF079106).