SB-mediated integration sites from the adenoviral vector in female C57Bl/6 mice using LM-PCR and LAM-PCR approaches.

<p>Chromosomal distribution of unique integration sites analyzed from female mice treated with functional AdV-SB hybrid vectors are compared to a computer-simulated random integration profile. (<b>a</b>) Integration events identified by LM-PCR. Percentages of integration events within each individual chromosome in comparison to the total number of identified integration sites are depicted on the upper panel. A schematic overview of chromosomal distribution is shown on the lower panel. Respective triangles indicate the relative positions of the chromosomal transposon integration site observed from liver genomic DNA of female mouse f1 (black triangles), female mouse f2 (grey triangles) and female mouse f3 (white triangles). (<b>b</b>) Sequence reads and chromosomal distribution identified after performing LAM-PCR from female mouse f1 and f2. Percentages of integration events within each individual chromosome in comparison to the total number of identified integration sites are depicted on the upper panel. The lower panel shows mapping of all 724 unique integration sites identified by LAM-PCR which were mapped within the mouse genome. Respective triangles indicate the relative positions of the chromosomal transposon integration site observed for liver genomic DNA of female mouse 1 (black triangles) and female mouse 2 (grey triangles).