Ansamitocin P3 induced apoptosis of MCF-7 cells.

<p>(A) The apoptosis in MCF-7 cells was estimated by Annexin V and PI staining followed by flow cytometry analysis. Quadrants represent dead (Q1), late apoptotic (Q2), live (Q3) and (Q4) early apoptotic cells. (B) Ansamitocin P3 treatment caused cleavage of PARP. Lanes 1, 2, 3 and 4 represent control, 100 and 150 pM ansamitocin P3 and 25 nM vinblastine treatment of MCF-7 cells respectively. β-actin was used as loading control.