Effects of ansamitocin P3 on cell proliferation and cell cycle progression.

<p>(A) Inhibition of MCF-7 (▪), HeLa (▴), EMT-6/AR1 (•) and MDA-MB-231(▾) cell proliferation by ansamitocin P3. (B) Exposure of MCF-7 cells to ansamitocin P3 for 24 h increased the percentage of cells in G2/M phase of the cell cycle. (C) Ansamitocin P3 treatment increased the percentage of phosphohistone [PH-H3 (S10)] stained cells. Scale bar is 10 µm. MCF-7 cells were incubated with vehicle (control) and different concentrations (20, 50 and 100 pM) of ansamitocin P3 for 24 h.