Effect of CO-OCS and SO-OCS on the expression of G1 and G2/M key proteins in MCF-7 cells.

<p>MCF-7 cells were treated with CO-OCS and SO-OCS (40 µM) for 72h. Cells were harvested at the indicated times and lysed in RIPA buffer. Cell lysates were analyzed by Western blotting using primary antibodies directed against CDK4, cyclin D1 (A), cyclin E (A), p21^Waf1/Cip1 (C), and cyclin B1 proteins (D). B, Effect of CO-OCS on phosphorylated Rb protein (ppRb) vs Rb protein (pRb). For each protein, a representative immunoblot of three independent experiments is shown (left panel). Protein levels were quantified and normalized to actin or pRb (right panel). The indicated values are the mean ± SD of three independent experiments (right panel). <i>^*p</i><0.05, <i>^** p<</i>0.01, <i>^*** p<</i>0.001.