Chemical crosslinking of hSVCT1 in isolated oocyte membranes.

<p>Western blot analysis of isolated oocyte membranes containing hSVCT1 incubated at different DST concentrations, i.e., 0, 100, 250, 500 and 1000 <i>µ</i>M. hSVCT1 monomer (<b>M</b>), dimer (<b>D</b>) and aggregate (<b>A</b>) bands are indicated. At ≥250 <i>µ</i>M DST increasing smearing (<b>S</b>) and aggregation, and decreasing amounts of monomers are observed indicating unspecific crosslinking. Western blotting was performed using a 6% SDS/polyacrylamide gel and an anti-HA antibody. The interface between stacking and separating gels is indicated (dotted, white line).