Effect of ghrelin (4 µM) on the mPSCs in the GnRH neurons of the female metestrous mice.

<p><b>a</b>) Ghrelin decreased the frequency of the mPSCs (N=14) with no change in the shape of the individual mPSCs (insets). Time course of instantaneous frequency is depicted under the mPSC-recording. <b>b</b>) Effect of ghrelin on the mPSCs was abolished by the pretreatment with AM251 (N=11). <b>c</b>) Intracellularly applied THL eliminated the ghrelin-evoked changes on the mPSCs (N=10). <b>d</b>) Bar graph reveals that ghrelin significantly diminished the frequency of mPSCs. Arrow shows the onset of ghrelin administration. *=p<0.05.