Effect of BV-4 fractions on NO generation and cell viability.

<p> In (A), RAW 264.7 macrophages (1×10⁶ in 2 ml of medium) were incubated for 30 min with or without the indicated concentrations of the neutral, acidic, or phenolic fraction of BV-4, then for 24 h with or without addition of 1 µg/ml of LPS, then NO generation in the culture medium was measured by the Griess reaction. In (B), RAW 264.7 macrophages (1×10⁶ in 2 ml of medium) were incubated for 30 min with or without the indicated concentration of the phenolic fraction of BV-4, then for 24 h with or without addition of 1 µg/ml of LPS, then NO generation in the culture medium was measured by the Griess reaction. In (C), RAW 264.7 macrophages (5×10⁴ in 1 ml of medium) were incubated for 30 min with or without the phenolic fraction of BV-4, then for 24 h with or without addition of 1 µg/ml of LPS, then cell viability was measured by the AlamarBlue® assay. The data are expressed as the mean ± SD for three separate experiments. *and # indicate a significant difference at the respective levels of <i>p</i><0.05 and <i>p</i><0.001 compared to the LPS-treated group.