Scoring of leukocyte migration in the transected tails of zebrafish larvae.

<p>All larvae (<i>nacre</i>) are four days post-fertilization (4 dpf), with anterior to the left, scale bar = 10 µm. Dark spots (marked by arrows) represent leukocytes migrating to the injured zone in the transected tails. Migrating leukocytes were counted in the region to the right of the dashed red arc. <b>A</b>, tail of an uncut larva; <b>B</b>, tail-cut with score 0; <b>C</b>, tail-cut with score 1; <b>D</b>, tail-cut with score 2; <b>E</b> tail-cut with score 3; <b>F,</b> tail-cut with score 4. <b>^a</b>Experimental values obtained for each larvae are normalized to a relative value expressed as relative leukocyte migration (RLM); +LPS: with inclusion of lipopolysaccharides. <b>^b</b>Percentage of anti-inflammatory activity is obtained as (1−RLM)x100.