Quantitative PCR validation of microarray results.
- Zhi-Qiang Wang (244034)
- Mamadou Keita (467069)
- Magdalena Bachvarova (74369)
- Stephane Gobeil (108928)
- Chantale Morin (467070)
- Marie Plante (21802)
- Jean Gregoire (467071)
- Marie-Claude Renaud (467072)
- Alexandra Sebastianelli (467073)
- Xuan Bich Trinh (347448)
- Dimcho Bachvarov (30717)
Publication date
October 2013
DOIAbstract
<p>The figure shows bar graphs presentation of the differential expression of the selected genes in the shRNA-RUNX2 clone 3 (cl-sh3) compared to the control clone. The relative copy number was calculated based on the target gene/18S ribosomal RNA ratio. Values more than or equal to 1 represent gene upregulation and more than 1 display gene downregulation. The analysis confirmed a higher level of PCDH9, DEFB1 expression and lower levels of TRPM8, MMP13, MMP1, IL1A, CXCL12, ALDH1A1, CFH, TUBB1 expressions in the cl-sh3 clone.</p
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