Role of NADPH oxidases in IH-induced HIF-2α degradation.

<p><b>A.</b> Effect of NADPH oxidase (Nox) inhibitors Apocynin (Apo, 1 mM) and AEBSF (15 µM) on HIF-2α protein following exposure to IH. <b>B.</b> HIF-2α expression in PC12 cells transfected with Nox2 and Nox4 siRNA and exposed to normoxia (N) or IH. Tubulin expression was monitored as control for protein loading. Bottom panels of A and B represent average data of densitometric analysis of the immunoblots presented as mean ± S.E.M from three independent experiments. *<i>p</i><0.05; n.s. not significant, <i>p</i>>0.05.