Functional analysis of GM-IMs.

<div><p>(A) Bacterial killing assay. <i>S. aureus</i> (left) or <i>E. coli</i> (right) were serially diluted in 96-well plates. They were mixed with 5 x 10⁴ PMφ or GM-IMs and cultured overnight in 100 µL RPMI-1640 and 10% FCS and 3% GM-CSF-CM. One µL of mixed culture at appropriate dilution (which showed the clear differences in the number of bacteria) was plated on an agar plate. After 24 hr, the number of colony forming units (CFU) was counted. Data shown are the mean ratios ± SE of three independent experiments. P value: * <0.01, **<0.001.</p> <p>(B) May-Grunwald-Giemsa stain of GM-IMs cultured with <i>E. coli</i> for 24 hr. Left shows GM-IMs without bacteria, and right shows GM-IMs with bacteria. Scale bars: 100 µm. Data are representative of three independent experiments.</p>