PDH Inhibition is Relieved and PDK4 Content Reduced Upon Removal of Mice from the High Fat Diet.

<p>C57BL/6 mice were fed either a control diet for 1 d, a high fat diet for 1 d, or a high fat diet for 1 d followed by a control diet for 1 d. <b>A</b>. Cardiac mitochondria were incubated with 100 µM pyruvate and 1.0 mM malate. State 3 respiration was initiated by the addition of ADP (0.25 mM). PDH activity was assayed during state 3 respiration (n = 3). <b>B</b>. Western blot analysis (representative of n = 3) and quantitative mass spectrometry (n = 3) were used to determine PDK4 protein levels in isolated cardiac mitochondria. All data are presented as the mean ± SEM with <i>p</i> values: * < 0.05; ** < 0.01; and *** < 0.001.