PDK4-Upregulation Occurs Prior to Reductions in GLUT4 Expression and Insulin-Stimulated Akt Phosphorylation.

<p>Mice were placed on a high fat or control diet for 3 d or 1 wk. Hearts were then processed and <b>A</b>. GLUT4 expression was determined by Western blot analysis and quantified by densitometric analysis (n = 5) and <b>B</b>. Cardiac insulin signaling was assessed as insulin-stimulated Akt phosphorylation, quantified by densitometric analysis (n = 5), and represented relative to total Akt. As indicated, wild type or PDK4 knockout mice were utilized. All data are presented as the mean ± SEM with <i>p</i> values: * < 0.05 and *** < 0.001.