High Dietary Fat Alters the Kinetics of PDH Inhibition in Wild-type but not PDK4-/- Mice.

<p>Cardiac mitochondria (0.25 mg/mL) from mice fed control or high fat diets for 1 wk were incubated with 100 µM pyruvate and 1.0 mM malate. At 2.0 min, state 3 respiration was initiated by the addition of 0.25 mM ADP. PDH activity was assayed at indicated time points for <b>A</b>. wild-type mice (n = 5) and <b>B</b>. PDK4^-/- mice (n = 5). <b>C</b>. Oxygen consumption traces for mitochondria respiring with pyruvate were recorded. The rate of state 3 respiration was calculated for wild-type mice (n = 11) and PDK4^-/- mice (n = 5). <b>D</b>. The relative levels of PDH E1α and Hsp60 as a loading control were assessed in wild-type and PDK4^-/- mice fed control or high fat diet by Western blot analysis. All data are presented as the mean ± SEM with <i>p</i> values: * < 0.05 and ** < 0.01.