SDS-PAGE analysis of archaeal membrane transport proteins in <i>E. coli</i> membranes.

<p>Membrane transport protein genes cloned into pTTQ18, pET52b and pWarf were tested in two <i>E. coli</i> host strains, C43(DE3) and BL21 Star. Membranes were analysed by SDS-PAGE. C - C43(DE3), S - BL21 Star, 1 - pTTQ18, 2 - pET52b(+), 3 - pWarf(-). Positions of the His-tagged protein determined by Western blotting (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0076913#pone-0076913-g003" target="_blank">Figure 3</a>) are indicated by: triangle - protein detected by Western blotting but not visible on SDS-PAGE; star - protein detected by Western blotting and visible on SDS-PAGE gels.