<p>All samples were run in duplicate (template added and template free). For further details about the position and size of the target, see supplemental material and methods.</p
<p>Real-time PCR were performed with 2 µM of primers at Tm 50°C. Cells filled in bold indicate the b...
<p>The primers were used for quantitative RT-PCR. Each primer pair binds specifically the indicated ...
<p>All sequences are given in the 5′ to 3′ direction. All Taqman probes were FAM-MGB labeled.</p
<p>Probes and primer sets were custom designed with 5’ labeled 6 FAM and 3’ labeled TAMRA.</p><p>Pri...
<p>The sequences of primers for quantitative real time RT-PCR used in this study.</p
<p>All plasmids, at 10<sup>−5</sup> dilution each, were tested at Tm 45°C. ‘C’: number of plasmid co...
<p>F, Forward primer; R, reverse primer. GAPDH as an internal standard <a href="http://www.plosone.o...
<p>Unique primers for each nAchR subunit were used for the relevant PCR reactions. All of the result...
<p>Sequences of primers used for RT-PCR are based on human genes and shown from 5′ to 3′.</p
<p>F<sup>a)</sup>, sequence from sense strands; R<sup>b)</sup>, sequence from anti-sense strands.</p
<p>Primers adopted for RT-PCR experiments as depicted in <a href="http://www.plosone.org/article/inf...
<p>(A) Sequencing data of amplified regions. Numbers on upper right corner indicate the highest cove...
<p>The sequences of the primers and the annealing temperature (AT) are shown for each gene under stu...
<p>The table shows sample size, primer combinations, annealing temperature and PCR cycle number as w...
<p>Primer sequences, fragment lengths and PCR conditions including number of cycles, annealing tempe...
<p>Real-time PCR were performed with 2 µM of primers at Tm 50°C. Cells filled in bold indicate the b...
<p>The primers were used for quantitative RT-PCR. Each primer pair binds specifically the indicated ...
<p>All sequences are given in the 5′ to 3′ direction. All Taqman probes were FAM-MGB labeled.</p
<p>Probes and primer sets were custom designed with 5’ labeled 6 FAM and 3’ labeled TAMRA.</p><p>Pri...
<p>The sequences of primers for quantitative real time RT-PCR used in this study.</p
<p>All plasmids, at 10<sup>−5</sup> dilution each, were tested at Tm 45°C. ‘C’: number of plasmid co...
<p>F, Forward primer; R, reverse primer. GAPDH as an internal standard <a href="http://www.plosone.o...
<p>Unique primers for each nAchR subunit were used for the relevant PCR reactions. All of the result...
<p>Sequences of primers used for RT-PCR are based on human genes and shown from 5′ to 3′.</p
<p>F<sup>a)</sup>, sequence from sense strands; R<sup>b)</sup>, sequence from anti-sense strands.</p
<p>Primers adopted for RT-PCR experiments as depicted in <a href="http://www.plosone.org/article/inf...
<p>(A) Sequencing data of amplified regions. Numbers on upper right corner indicate the highest cove...
<p>The sequences of the primers and the annealing temperature (AT) are shown for each gene under stu...
<p>The table shows sample size, primer combinations, annealing temperature and PCR cycle number as w...
<p>Primer sequences, fragment lengths and PCR conditions including number of cycles, annealing tempe...
<p>Real-time PCR were performed with 2 µM of primers at Tm 50°C. Cells filled in bold indicate the b...
<p>The primers were used for quantitative RT-PCR. Each primer pair binds specifically the indicated ...
<p>All sequences are given in the 5′ to 3′ direction. All Taqman probes were FAM-MGB labeled.</p
DOI
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