Cldn6 triggers epithelial differentiation in mouse F9 stem cells.

<p>(A) Western blot showing expression of Cldn6 protein in 10 clones of F9:Cldn6 cells and control F9 cells. (B and C) Morphological appearance and localization of Cldn6, ZO-1 and E-cadherin (E-Cad) in control F9 and F9:Cldn6 cells. Scale bars, (A) 50 µm; (B) 20 µm. (D) RT-PCR for the indicated molecules in 4 clones of F9:Cldn6 cells and F9 L32T2:HNF4α cells treated for 72 h with the vehicle or 1.0 µg/ml doxycycline (Dox). Quantification of the mRNA levels is shown in the histograms (mean+SD; <i>n</i> = 4). *<i>P</i><0.05; **<i>P</i><0.001 compared with values of the lane 5. (E) Western blot for the indicated molecules in F9:Cldn6 cells and F9 L32T2:HNF4α cells treated for 72 h with the vehicle or 1.0 µg/ml Dox. Quantification of the protein levels is shown in the histograms.