Transposition efficiency of the AAV/SB transposase hybrid-vector system.

<div><p>Colony forming assay to determine integration efficiencies from the hybrid AAV/SB vector system in HeLa-cells. HeLa-cells were co-infected with AAV-neo and AAV-SB100X at increasing dosages (MOI 100, 1,000, 10,000 and 50,000).</p> <p>(<b>a</b>) The AAV vector AAV-SB100X contains a transgene expression cassette for the hyperactive Sleeping Beauty (SB) transposases SB100X expressed under the control of the cytomegalovirus promoter (CMV). The control vector AAV-mSB encodes the mutated and inactive version of SB (mSB).</p> <p>(<b>b</b>) After co-transduction and selection, obtained cell colonies were either collected as pools for integration site analyses or colonies were stained with methylene blue and counted to determine integration efficiencies.</p> <p>(<b>c</b>) Result of the colony forming assay. The Y-axis shows the number of neomycin-resistant colonies obtained in different experimental settings. Error bars indicate standard deviation (n=3). n.d.: not determined.</p>