Transcriptional response to vitamin D overall and for specific vitamin D responsive genes.

<p>(<b>a</b>) Volcano plot of transcriptional response to vitamin D at 8 hours and 24 hours respectively summarizing mean log₂ fold change (vitamin D/vehicle) and corresponding evidence of differential expression (-log₁₀ p-value) for each gene shown as a single point. The red line corresponds to an FDR of 1%. (<b>b</b>) Boxplots showing examples of two established vitamin D responsive genes at 24 hours including <i>CYP24A1</i> and <i>CAMP</i>. <i>CYP24A1</i> encodes 1,25-dihydroxyvitamin D 24-hydroxylase, a member of the cytochrome P450 superfamily, that initiates degradation of 1,25 vitamin D and had the largest response to vitamin D treatment (log₂ fold change 4.54, p-value=2.40 x 10^-9). <i>CAMP</i>, cathelicidin anti-microbial peptide, is a known target of the vitamin D receptor and also showed a large response to treatment (log₂ fold change 2.62, p-value=1.64 x 10^-7).