TEL-Syk chimeric mice developed bone marrow hypocellularity and fibrosis.

<p>(A) Numbers of bone marrow cells isolated from mice receiving vector, Syk, TEL-Syk KD and TEL-Syk transduced fetal liver hematopoietic cells at 60 days following cell transfer. Data are shown as mean ± SEM for vector (n=14), Syk (n=13), TEL-Syk (n=7), TEL-Syk KD (n=14). Flow cytometric analysis of (B) leukocytes percentages and (C) total leukocyte numbers in the bone marrow cells. (D, E) H&E stained sternum sections from vector and TEL-Syk expressing mice, (F, G) Wright-Giemsa staining of bone marrow smears, and (H, I) cytospins of bone marrow cells isolated from femurs and tibias of TEL-Syk and vector expressing mice at 60 days following cell transfer. Dysplastic megakaryocytes (arrow) and neutrophils (asterisk) are indicated. (J, K) Reticulin-stained sternum sections from vector and TEL-Syk expressing mice. Scale bars correspond to 30 µm (D-G, J, K) and 10 µm (H, I). Data is shown as mean ± SEM and statistical significance was determined by one-way ANOVA. *P< 0.05, ***P< 0.001.