The effect of MGSTA-5 and MGSTA-6 on migration of canine mammary carcinoma cells evaluated by trans-well migration assay

<p>(A) Quantification of migration of examined cell lines (CMT-W1, CMT-W1M, CMT-W2, CMT-W2M) is presented as Relative Florescent Units (RFU) obtained using florescent plate reader Infinite 200 PRO Tecan™ (Ex. 485, Em. 530). The treatment of cancer cells with MGSTA-5 and MGSTA-6 for 20 hrs at the concentration of 100 µM decreased the migration through membrane of CMT-W1, and CMT-W1M cells and almost completely abolished migration of CMT-W2 cells. In CMT-W2M cell line those compounds were ineffective. The experiment was performed three times. The statistical analysis was done using Prism version 5.00 software (GraphPad Software, USA). One-way ANOVA followed by Tukey HSD post-hoc test and unpaired t-test were applied. p < 0.05 was marked as *, p < 0.001 was marked as ***. (B) Micrographs of the migrated CMT-W1, CMT-W1M and CMT-W2 cells taken with Olympus microscopy BX60 at x4 magnification.