miR-26a overexpression inhibited pancreatic cancer cell growth by the downregulation of cyclin E2 expression.

<p>The qRT-PCR analysis demonstrated the transcription of miR-26a in mimics, inhibitor, and control groups (A,B,C), and the expression of cyclin E2 in cyclin E2 siRNA, control siRNA, and control groups (D,E,F). The proliferation of PDAC cell lines transiently transfected with miR-26a mimics, miR26a inhibitor or cyclin E2 siRNA was analyzed by the CCK-8 proliferation assay (G,H,I). The regulation of cyclin E2 or EZH2 expression by miR-26a was analyzed by Western blot (J,K,L), and Western blot analysis also confirmed the expected efficiency of cyclin E2 siRNA in three PDAC cell lines (M,N,O).