The genetic signature of within breed sub-type on chromosome 13.

<p>Individuals representing the extremes of physical appearance (Meatmouth, heavily wrinkled thickened skin, <i>n</i> = 123; Bonemouth, few wrinkles left into adulthood, <i>n</i> = 33) were compared. <b>A</b>. Pairwise <i>F</i>_ST was calculated between the two groups for 126,206 SNP and plotted in chromosomal order. Smoothed values in bins of 100 SNP are superimposed to better illustrate the peak of association (red line). <b>B</b>. A zoomed view of a subset of the genes from the chromosome region in relation to the peak of <i>F</i>_ST (based on most divergent SNP and a 5% threshold, red box). <b>C</b>. The plot of a mixed model association test for breed subtype. The top genome-wide significant SNP (13∶23180227, <i>p_MM</i> = 2.63×10⁻⁹) has been highlighted with an open circle and linkage disequilibrium (<i>r²</i>) between this marker and the others in the region are coloured according to strength. A genome wide significant threshold (red dotted line) has been defined using an analysis specific Bonferroni 5% limit.