PPADS inhibits formation of tubes by HUVECs.

<p><b>A</b>) Representative phase contrast images of tubes formed in the absence of supplements (negative control), in the presence of vehicle (0.9% NaCl) or vehicle plus 1.04 µM PPADS or 4.17 µM PPADS. <b>B</b>) Tabular summary of mean values ± SEM of master junctions, master segments and meshes in cells incubated with the indicated concentration of PPADS and calculated IC₅₀ for each marker. <b>C</b>) Dose dependent PPADS mediated reduction of master junctions, master segments and meshes. The punctuated line indicates the extrapolated inhibition trend. IC₅₀ values are indicated as intercept points in the trend line. Asterisks in each inset mark an example of a measured junction, segment or mesh, respectively. Studies were performed a total of 4 times in triplicate.