<p>(A) LoxP sites were introduced in the third and fourth introns of the <i>Vps35</i> gene by homologous recombination. (B) Southern blot and PCR analysis showing correct targeting of selected ES cell clone. </p
<p>A) To delete exons 2 and 3 in the endogenous <i>Tardbp</i> we used a targeting vector with 6 Kb a...
<p>(A) Targeting construct. Dark boxes: Exons; dark triangles: loxP sites. A mcl-neo cassette flanke...
<p><b>A</b>) The targeting vector construction is depicted schematically to show positions of the ke...
<p>A: Scheme of the targeting vector and of the Rosa26 locus following homologous recombination. “B”...
Abstract Background Conditional knockout mice are a useful tool to study the function of gene produc...
<p>(A) Targeting strategy for generating <i>Pou4f1</i> conditional null allele. (B) Targeting strate...
<p><b>(A)</b> Scheme of the wild-type allele, the targeting vector, and the targeted CAP2/<i>Tmprss4...
<p>(<b>A</b>) Targeting construct for <i>Tmem26</i> conditional inactivation compared to the wild ty...
<p>A- genomic locus MALPY00360 coding for <i>py01365</i> showing the two...
<p>(A) shows the targeting vector used to replace the 5′ flanking region of the mouse <i>Avpr1a</i> ...
<p>(<b>A</b>) Gene targeting in ES cells. A Southern blotting strategy was used to screen the mutant...
<p><b>A</b>. Schematic illustration of gene targeting and Southern strategies. Black lines and blue ...
Targeted gene mutation in the mouse is a primary strategy to understand gene function and relation t...
<p><b>(A)</b> Insertion of the targeting vector into the <i>Wwox</i> allele introduced a new Bgl II ...
<p>(<b>A</b>) Shows the scheme of the linearized targeting vector used for electroporation in mouse ...
<p>A) To delete exons 2 and 3 in the endogenous <i>Tardbp</i> we used a targeting vector with 6 Kb a...
<p>(A) Targeting construct. Dark boxes: Exons; dark triangles: loxP sites. A mcl-neo cassette flanke...
<p><b>A</b>) The targeting vector construction is depicted schematically to show positions of the ke...
<p>A: Scheme of the targeting vector and of the Rosa26 locus following homologous recombination. “B”...
Abstract Background Conditional knockout mice are a useful tool to study the function of gene produc...
<p>(A) Targeting strategy for generating <i>Pou4f1</i> conditional null allele. (B) Targeting strate...
<p><b>(A)</b> Scheme of the wild-type allele, the targeting vector, and the targeted CAP2/<i>Tmprss4...
<p>(<b>A</b>) Targeting construct for <i>Tmem26</i> conditional inactivation compared to the wild ty...
<p>A- genomic locus MALPY00360 coding for <i>py01365</i> showing the two...
<p>(A) shows the targeting vector used to replace the 5′ flanking region of the mouse <i>Avpr1a</i> ...
<p>(<b>A</b>) Gene targeting in ES cells. A Southern blotting strategy was used to screen the mutant...
<p><b>A</b>. Schematic illustration of gene targeting and Southern strategies. Black lines and blue ...
Targeted gene mutation in the mouse is a primary strategy to understand gene function and relation t...
<p><b>(A)</b> Insertion of the targeting vector into the <i>Wwox</i> allele introduced a new Bgl II ...
<p>(<b>A</b>) Shows the scheme of the linearized targeting vector used for electroporation in mouse ...
<p>A) To delete exons 2 and 3 in the endogenous <i>Tardbp</i> we used a targeting vector with 6 Kb a...
<p>(A) Targeting construct. Dark boxes: Exons; dark triangles: loxP sites. A mcl-neo cassette flanke...
<p><b>A</b>) The targeting vector construction is depicted schematically to show positions of the ke...
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