Validation of microarray data using quantitative PCR.

<p>Expression levels of DMB genes found to be up regulated (red; top and center rows) or down regulated (green; bottom row) on microarrays were determined with the assistance of qPCR (ΔΔCT method) in human (circle) and mouse (square) lymphoma (open) counterparts. Normal B cells (closed) were included for comparison. <i>HPRT1</i> and <i>Hprt</i> were used as internal reference genes for human and mouse samples, respectively. Median gene expression levels are indicated by horizontal lines. Statistical analysis relied on the Mann-Whitney test.