<p>The empty pURE2 plasmid (Mock) or pURE2-Vp1 plasmids encoding either WT Vp1 or cysteine point mutant Vp1s were subjected to cell-free transcription-coupled translation, and the translation products were examined for Vp1 by SDS-PAGE and immunoblotting.</p
AbstractHIV reverse transcriptases (RTs) have few cysteine residues relative to other RTs and retain...
Viral RNA synthesis was assayed in HeLa cells transfected with nonviable poliovirus RNA mutated in t...
Origin of supplementary truncated VP1 protein band (signaled on the figure by *) was studied by West...
<p>(A) Schematic diagram of cysteine point mutant Vp1s. Each of the six cysteine residues in the 354...
<p>(<b>A</b>) 293T cells were cotransfected with HIV-1 proviral (pNL4-3) plasmids expressing various...
<p>(A) HeLa cells transfected with an empty plasmid (Mock) or plasmid encoding WT, C80A, or C247A Vp...
<p>HeLa cells were transfected with 0.5 ug DNA of empty vector (Ctrl), FLAG-tagged VPS11-WT or VPS11...
(A) Expression of wild type Vps1GFP and Vps1(KKK-AAA)-GFP mutant in cells. Western blot showing simi...
<p>A) Equal amounts of various pCMV HA Vpu expression constructs were transfected into HEK-293T cell...
<p>(A) The SV40 VP1 gene was cloned from SV40 WT or VPm using PCR and then inserted into an expressi...
<p>Cell lines modified to contain a single copy of the wild type (CCGAC) or cysteine mutant <i>GPI-P...
Structural studies have implicated Cys9, Cys104 and Cys207 of simian virus 40 (SV40) Vp1 in disulfid...
<p>(<b>A</b>) 293T cells were co-transfected with pcDNA3-hVpr plasmids expressing WT or mutant Vpr (...
(A) Polyprotein products of in vitro translation of wild type and M1-M6 mutants of CVB3 in cell-free...
<p>(A) Alignment of amino acid sequences of JCV, SV40, BKV, and MPyV Vp1s in the vicinity of C80. Am...
AbstractHIV reverse transcriptases (RTs) have few cysteine residues relative to other RTs and retain...
Viral RNA synthesis was assayed in HeLa cells transfected with nonviable poliovirus RNA mutated in t...
Origin of supplementary truncated VP1 protein band (signaled on the figure by *) was studied by West...
<p>(A) Schematic diagram of cysteine point mutant Vp1s. Each of the six cysteine residues in the 354...
<p>(<b>A</b>) 293T cells were cotransfected with HIV-1 proviral (pNL4-3) plasmids expressing various...
<p>(A) HeLa cells transfected with an empty plasmid (Mock) or plasmid encoding WT, C80A, or C247A Vp...
<p>HeLa cells were transfected with 0.5 ug DNA of empty vector (Ctrl), FLAG-tagged VPS11-WT or VPS11...
(A) Expression of wild type Vps1GFP and Vps1(KKK-AAA)-GFP mutant in cells. Western blot showing simi...
<p>A) Equal amounts of various pCMV HA Vpu expression constructs were transfected into HEK-293T cell...
<p>(A) The SV40 VP1 gene was cloned from SV40 WT or VPm using PCR and then inserted into an expressi...
<p>Cell lines modified to contain a single copy of the wild type (CCGAC) or cysteine mutant <i>GPI-P...
Structural studies have implicated Cys9, Cys104 and Cys207 of simian virus 40 (SV40) Vp1 in disulfid...
<p>(<b>A</b>) 293T cells were co-transfected with pcDNA3-hVpr plasmids expressing WT or mutant Vpr (...
(A) Polyprotein products of in vitro translation of wild type and M1-M6 mutants of CVB3 in cell-free...
<p>(A) Alignment of amino acid sequences of JCV, SV40, BKV, and MPyV Vp1s in the vicinity of C80. Am...
AbstractHIV reverse transcriptases (RTs) have few cysteine residues relative to other RTs and retain...
Viral RNA synthesis was assayed in HeLa cells transfected with nonviable poliovirus RNA mutated in t...
Origin of supplementary truncated VP1 protein band (signaled on the figure by *) was studied by West...