Immunohistochemical analyses of autophagy cascade in sedentary and exercised VCP^R155H/+ mice.

<p>Immunohistochemical analysis of sedentary and exercised WT and VCP^R155H/+ mice stained with (A,B) Ubiquitin, (C,D) P62/SQSTM1, (E,F) COXIV, (G,H) LC3-I/II, (I,J) Ubiquitin and TDP-43 (arrows pointing to TDP-43 localization) (Magnification: 630X). (K) Western blot and (L) densitometry analyses of these proteins demonstrated lower levels of LC3-I/II, Ubiquitin, P62/<i>SQSTM1</i>, and TDP-43 upon the uphill exercise regimen in VCP^R155H/+ animals (Magnification: 630X). Statistical significance is denoted by *<i>p</i><0.05, **<i>p</i><0.005, and ***<i>p</i><0.001. The number of animals used was n = 8–10/group.