Androgen-stimulated PAK6 activation is mediated by androgen receptor. LAPC4 cells were plated overnight in IMDM supplemented with 10% charcoal-stripped fetal bovine serum.

<p>(A) Time course (0 h: non-treated) study of PAK6 activation in response to 10 nM dihydrotestosterone (DHT) stimulation in LAPC4 cells. (B) A DHT ten-fold serial dilution (0 nM: non-treated; and DHT-treated 0.1 nM to 1,000 nM) dose response of PAK6 activation. (C) 10 nM DHT does not activate PAK6 kinase activity in androgen receptor negative PC3-PAK6 cell. (D) PAK6 <i>in </i><i>vitro</i> kinase assay demonstrates knocking down of AR by siRNA diminishes androgen-stimulated PAK6 activation in LAPC4 cells. Middle panels depict the expression levels of AR and PAK6 in LNCap-PAK6 cells in response to AR specific siRNA. (E) Whole lysate of LNCap, LAPC4 and PC3 blot against anti-PAK6,anti-AR and anti-actin.