Analysis of IL-10 induction by IL-27 in melanoma.

<p>(A) Constitutive expression of IL-27Rα and gp130 was analyzed by FACS on 4 melanoma cell lines as indicated. (B) Expression of <i>IL-10</i> was analyzed by qPCR in the A375 melanoma cell line cultured for 3 days in the presence or absence of IL-27 or TGF-ß1. (C) Supernatants from the A375 cell line cultured for 3 and 4 days in the presence or absence of IL-27 or TGF-ß1 were tested for IL-10 by ELISA. A representative experiment of 3 is shown in (B) and (C). (D) Distribution of cases that expressed IL-10 or not, among IL-27-negative or -positive cutaneous and metastatic melanomas. (E) Staining for IL-10 in a case of a primary cutaneous melanoma and a case of metastatic melanoma that were positive for IL-27. The bar represents 100 µM.