Mutants affected in Rad53 and Bfa1 display different phenotypes.

<p>(A–B) <i>cdc13-1</i> (F965), <i>cdc13-1 rad53-21</i> (F1228), <i>cdc13-1 bfa1</i>Δ (F1023) and <i>cdc13-1 rad53-21 bfa1</i>Δ (F1229) cells were grown in YPD at 23°C and arrested in G1 with pheromone. (A) Cells were then released into YPD at 34°C and the percentages of large budded and rebudded cells were determined at the indicated time points. Error bars indicate SD (n = 3). (B) Representative images of rebudded cells (DIC) and the nuclear morphology (DAPI) are also presented. (C) Wild type (F1587), <i>cdc13-1</i> (F965), <i>cdc13-1 rad53-21</i> (F1228) and <i>cdc13-1 bfa1</i>Δ (F1023) cells were plated by spotting 10-fold serial dilutions of a liquid culture (OD₆₀₀ = 0.3) on YPD and then incubated at 23, 27 or 30°C.