H3.1 is minimally mobilized in U2OS cells.

<p>(<b>A</b>) Average normalized levels of free GFP-H3.1 relative to mock-infected cells at 4 or 7 hpi, respectively. U2OS cells were transfected with plasmids expressing GFP-H3.1. Transfected cells were mock-infected or infected with 30 PFU/cell of strain KM110 (<b>KM110</b>) or 6 PFU/cell of strain KOS (<b>KOS</b>). Mobilization of GFP-H3.1 was examined from 4 to 5 (<b>4</b>) or 7 to 8 (<b>7</b>) hpi by FRAP; error bars, SEM; dashed line, average normalized levels of free GFP-H3.1 in mock-infected cells. (<b>B</b>) Frequency distribution plots of the percentage of free GFP-H3.1 per individual cell at 4 or 7 hpi; dotted line, one SD above the average level of free GFP-H3.1 in mock-infected cells. (<b>C</b>) Average initial rates of normalized fluorescence recovery relative to mock-infected cells at 4 hpi; error bars, SEM. (<b>D</b>) Frequency distribution plots of the initial rate of normalized fluorescence recovery of GFP-H3.1 per individual cell; dotted line, one SD above (KM110) or below (KOS) the average initial rate of normalized fluorescence recovery in mock-infected cells. **, P<0.01; <i>n.s.</i>, not significant.