Restriction of HIV-1 infection by Trim5-NUP153_C fusion proteins.

<p>(<b>A</b>) Schematic of Trim-NUP153_C fusion and control constructs. Color code: Trim5 RBCC, brown; rhTrim5α SPRY, auburn; HA-tag, blue; NUP153_C, orange. (<b>B</b>) Western blot of HOS cells stably transduced with HA-tagged Trim-NUP153_C fusion or control constructs, detected with antibody 3F10. (<b>C</b>) Western-blot detection of Trim-NUP153_C fusion proteins with anti-HA monoclonal antibodies 3F10 and 16b12. Antibody 3F10 detects full-length Trim-NUP153_C-HA whereas antibody 16b12 more faithfully detects full-length Trim-HA-NUP153_C. (<b>D</b>) Infectivity of various doses of HIV-1 (left) or MLV (right) GFP reporter viruses on HOS cells stably expressing various Trim-based constructs. The results are an average of two experiments, with error bars denoting standard error. Asterisks in panels B and C mark bands that correspond to the expected mobilities of full length Trim-NUP153_C constructs.