CCR2 deficiency inhibits bone marrow-derived myofibroblast formation in obstructive nephropathy.

<p><b>A</b>. Representative photomicrographs of CD45 and α-SMA immunostaining in the kidneys of WT and CCR-KO mice 2 weeks after UUO. <b>B</b>. Quantitative measurements of α-SMA protein expression in the kidneys of WT and CCR2-KO mice 4 weeks after UUO. ** <i>P</i> <0.01 vs WT controls; ^##<i>P</i> <0.01 vs WT UUO; ⁺<i>P</i> <0.05 vs KO UUO. n=6 per group. <b>C</b>. Representative photomicrographs of CD45 and FSP-1 immunostaining in the kidneys of WT and CCR-KO mice 2 weeks after UUO. <b>D</b>. Quantitative measurements of FSP-1 protein expression in the kidneys of WT and CCR2-KO mice 2 weeks after UUO. ** <i>P</i> <0.01 vs WT controls; ^#<i>P</i> <0.05 vs WT UUO; ⁺<i>P</i> <0.05 vs KO UUO. n=6 per group. <b>E</b>. Representative Western blots show the levels of α-SMA protein expression in the kidneys of WT and CCR2-KO mice. <b>F</b>. Quantitative analysis of α-SMA protein expression in the kidneys of WT and CCR2-KO mice. ** <i>P</i> <0.01 vs WT controls; ^#<i>P</i> <0.05 vs WT UUO; ⁺<i>P</i> <0.05 vs KO UUO. n=5 per group. <b>G</b>. Representative Western blots show the levels of FSP-1 protein expression in the kidneys of WT and CCR2-KO mice. <b>H</b>. Quantitative analysis of FSP-1 protein expression in the kidneys of WT and CCR2-KO mice. * <i>P</i> <0.05 vs WT controls; ^#<i>P</i> <0.05 vs WT UUO; ⁺<i>P</i> <0.05 vs KO UUO. n=5 per group.