Inhibition of Cdc42 or TCL by siRNA delayed Non-traumatic cell migration.

<p>A. Non-traumatic cell migration assays performed on human corneal epithelial cells transfected with siRNA. Migration of cells transfected with siRNA targeting Cdc42 or TCL was delayed compared to control siRNA transfected cells. B. Western blot showed that Cdc42 or TCL were inhibited efficiently by individual siRNA 48hrs after transfection. The ratio of Cdc42 and TCL to loading control were shown. C. Chart of the averaged wound size change against time. Results shown are for siRNA transfected non-traumatic cell migration assay over 12 hours. Wound closure rate in cells transfected with siRNA targeting Cdc42 were significantly slower than control cells. *p<0.05.