Inhibition of Cdc42 or TCL by dominant-negative plasmids delayed HCET cell migration.

<p>A. Transfection efficiency of control plasmids and dominant negative plasmids were similar when evaluated by observing GFP under fluorescence microscope. B. <i>In </i><i>vitro</i> scratch wounding assay performed on human corneal epithelial cells transfected with Cdc42, TCL, TC10 or Chp dominant-negative plasmids. Control plasmid transfected cells and cells transfected with TC10, Chp dominant-negative plasmids closed wound faster than Cdc42 or TCL dominant-negative plasmids transfected cells. C. Non-traumatic cell migration assay performed on human corneal epithelial cells transfected with Cdc42, TCL, TC10 or Chp dominant-negative plasmids. Results were similar with scratch wounding assay.