Summary of HCMV specific IFNγ secreting T cell response.

<p>Whole PBMC or PBMC depleted of either CD4⁺ or CD8⁺ T cells from 17 HCMV seropositive donors were stimulated with overlapping peptide pools spanning the HCMV ORFs pp65, IE, gB, UL138 and LUNA. Cells were incubated for 48 hours and IFNγ production was detected by ELISPOT. Post incubation IFNγ spot forming units (SFU) were enumerated and background levels of IFNγ determined from a non-peptide stimulated control were subtracted from each value and converted into SFU/10⁶ cells.