Tumor growth and body weight of the orthotopic breast cancer model mice treated with IR (4 Gy) or SAHA (25 mg/kg×9) alone or in combination.

<p>(A) Body weight in Balb/c mice measured once per week. (B) The 4T1-luc cells were injected into mammary fat pads of Balb/c mice, observed for luciferase signals and photographed using IVIS 200. (C) Quantification of the luciferase signals. *, <i>p</i><0.05, versus control. (D) IHC staining of the mouse orthotopic tumor tissues. IHC was used to determine the expression levels of LC3 and γH2AX (×100 objective magnification). The percentage of LC3 and γH2AX-positive cells was determined using HistoQuest software (TissueGnostics).