BMDM acquire F11r expression following brain infiltration.

<p>(<b>A</b>) GVHD was induced in recipient BALB/cJ mice following total body irradiation (TBI) and bone marrow transplantation (BMT) from T cell depleted (TCD) B6.SJL-<i>Ptprc</i>^<i>a</i><i>Pepc^b/BoyJ</i> donors. Following C57BL/6J mouse donor lymphocyte infusions (DLI), immune cell infiltration was assessed by flow cytometry at 1 week, 2 weeks, and 3 weeks post-DLI (n=6 GVHD and n=6 BMT-only control per time point). (<b>B</b>) Control BMT-only mice do not exhibit GVHD and lack substantial macrophage (R3) or lymphocyte (R1, and R4 in Figure S4) infiltration. There is a very small CD11b⁺, CD45.1^high monocyte population that expresses F11r in the control brain (<2%; R3, right panel). (<b>C</b>) Chimeric mice with GVHD have donor monocyte infiltration (CD11b⁺, CD45.1^high cells; R3) with negligible CD11b⁺, CD45.1^low cells (R2). F11r and Sell expression (right column) of positively-labeled infiltrating donor monocytes (R3) over the course of 3 weeks of GVHD demonstrates a shift from F11r⁺ Sell⁺ cells to F11r⁺ only cells. (D) Bar graphs illustrate the mean and SEM for each population of immune system cells over the course of the three weeks following DLI.