Anti-LT antibody titration from serum, fecal suspension, and intestine wash samples of the IP and IN immunized mice.

<p>Panel A: anti-LT IgG and IgA antibody titers from serum and intestine wash samples of the IP immunized (solid dots) and control mice (circles) (p<0.01). Panel B: anti-LT IgG and IgA antibody titers from serum samples of the mice IN immunized with 130 µg fusion antigen with 2 µg CT adjuvant or 2 µg CT adjuvant alone, but not of mice immunized with 130 µg fusion antigen without CT or Tris-HCl buffer only. Panel C: anti-LT IgA antibody titers detected from fecal suspension samples of mice IN immunized with the fusion antigen with 2 µg CT adjuvant or CT adjuvant alone, but not of mice immunized with fusion antigen without CT or Tris-HCl buffer. Panel D: anti-LT IgG and IgA antibody titers detected in intestinal wash samples of the mice IN immunized with the fusion and CT or CT, but not with the fusion alone or Tri-HCl buffer. Anti-LT antibody titration ELISAs used 25 ng CT (Sigma) to coat each well of an Immulon 2HB plate (Thermo Scientific); HRP-conjugated goat-anti-mouse IgG (1:5000) or IgA (1:1000) as the secondary antibodies. Optical densities of greater than 0.4 (after subtracting the background reading) were used to calculate anti-LT antibody titers (in log₁₀).