Effects of GCN2 deletion on eIF2α phosphorylation pathways in 5XFAD mice.

<p>(A, C, D) Representative immunoblots of protein extracts from hippocampal homogenates of mice. (B, E) Immunoreactive bands were quantified and expressed as the percentage of 5XFAD control mice (<i>n</i> = 5–12). Note that GCN2 deficiencies aggravate eIF2α phosphorylation without affecting total eIF2α levels in 5XFAD mice. Moreover, GCN2 deletion induces overactivation of another eIF2α kinase PERK in 5XFAD mice, while PKR activities are not changed. *<i>p</i><0.05 vs. wild-type, ^#<i>p</i><0.05 vs. 5XFAD. All data are presented as mean ± SEM.