a<p>Forward and reverse primers are represented by plus (+) or minus (−), respectively.</p>b<p>restriction sites are italicized.</p>c<p>The bases mutated from those present in the wild type are bold.</p
a<p>Nucleotides in bold indicate restriction sites introduced for cloning.</p>b<p>Nucleotides in low...
<p>Strains, plasmids and primers used in this study. Where applicable restriction sites are underlin...
a<p>Ap<sup>R</sup>, Cm<sup>R</sup>, Km<sup>R</sup>, Gm<sup>R</sup> and Tc<sup>R</sup> indicate resis...
a<p>The bold letters denote the restriction sites.</p><p>Bacterial strains and plasmids used in this...
<p>Note: <sup>1</sup>The sequences in bold print are the integrated restriction enzyme sites.</p
a<p>Underline indicates the recognition sites by endonuclease.</p>b<p>Italic indicates the annealing...
<p>Restriction enzyme sites are boldfaced; added ATG or TAA codons are underlined; mutated nucleotid...
a<p>Underlined sequences are complementary to primers used for amplification of antibiotic resistant...
<p>Nucleotides of the primers that differ from the wild type sequence are presented in bold.</p
a<p>: The lower-case letters indicate the corresponding protective bases of the restriction enzymes....
<p>Wt: Wild-type; Mut: mutant; F: Forward primer; R: Reverse primer. The sequences of restriction si...
<p>Restriction enzyme sites generated by site-directed mutagenesis or by PCR are boldfaced and under...
<p>Note: Kan<sup>r</sup> and Spc<sup>r</sup>, for kanamycin and spectinomycin resistance, respective...
<p>Bacterial strains, plasmids and oligonucleotide primers used in this study.</p
<p>Underlined sequences indicate restriction enzyme sites. Bold indicates mutated sequences.</p
a<p>Nucleotides in bold indicate restriction sites introduced for cloning.</p>b<p>Nucleotides in low...
<p>Strains, plasmids and primers used in this study. Where applicable restriction sites are underlin...
a<p>Ap<sup>R</sup>, Cm<sup>R</sup>, Km<sup>R</sup>, Gm<sup>R</sup> and Tc<sup>R</sup> indicate resis...
a<p>The bold letters denote the restriction sites.</p><p>Bacterial strains and plasmids used in this...
<p>Note: <sup>1</sup>The sequences in bold print are the integrated restriction enzyme sites.</p
a<p>Underline indicates the recognition sites by endonuclease.</p>b<p>Italic indicates the annealing...
<p>Restriction enzyme sites are boldfaced; added ATG or TAA codons are underlined; mutated nucleotid...
a<p>Underlined sequences are complementary to primers used for amplification of antibiotic resistant...
<p>Nucleotides of the primers that differ from the wild type sequence are presented in bold.</p
a<p>: The lower-case letters indicate the corresponding protective bases of the restriction enzymes....
<p>Wt: Wild-type; Mut: mutant; F: Forward primer; R: Reverse primer. The sequences of restriction si...
<p>Restriction enzyme sites generated by site-directed mutagenesis or by PCR are boldfaced and under...
<p>Note: Kan<sup>r</sup> and Spc<sup>r</sup>, for kanamycin and spectinomycin resistance, respective...
<p>Bacterial strains, plasmids and oligonucleotide primers used in this study.</p
<p>Underlined sequences indicate restriction enzyme sites. Bold indicates mutated sequences.</p
a<p>Nucleotides in bold indicate restriction sites introduced for cloning.</p>b<p>Nucleotides in low...
<p>Strains, plasmids and primers used in this study. Where applicable restriction sites are underlin...
a<p>Ap<sup>R</sup>, Cm<sup>R</sup>, Km<sup>R</sup>, Gm<sup>R</sup> and Tc<sup>R</sup> indicate resis...