Proof-of-principle experiment using the WRKY11 DNA-binding domain (DBD).

<p>(A) Two individual DPI-ELISA screens were performed with WRKY11 DBD; a plate scan of replicate #2 is shown. (B) All 384 probes of the plate replicate #2 were hierarchically ranked according to their relative normalized absorbance values. : positive and negative controls, : dsDNA library probes, ???: indicate positive probes. Background shading indicates confidence interval (p = 0.05) (C) Comparison of the two biological replicate DPI-ELISA screens with WRKY11 DBD. Relative normalized absorbance values of replicate #1 (x-axis) and replicate #2 (y-axis) were plotted against each other to demonstrate the high reproducibility (left). : positive and negative controls, : dsDNA library probe, : indicate positive probes. <b>- - -</b>: borders of significance (p≤0.05). Probe name, plate position and rank of positively bound dsDNA library probes that clustered with the positive control probes are given for replicates #1 and #2 (right). (D) The positive dsDNA probe sequences were aligned according to DREME <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075177#pone.0075177-Bailey1" target="_blank">[54]</a>. Pos.: plate position. Finally, the DNA-binding consensus was derived (E).