Ultrastructural analysis of stably dominant negative DD-Hub expressing parasites.

<p>Parasites were cultured in absence (<b>A</b>) or presence (<b>B</b>-<b>E</b>) of Shield-1 for 24 hrs. (<b>A</b>) Section through a parasitophorous vacuole containing two daughters each showing normal morphology with nucleus (N), Golgi body (G) and apical organelles. C, conoid; R, rhoptries; M, micronemes; DG, dense granules. Note the small residual body (RB) is free of organelles. Bar is 1 µm. (<b>B</b>) Section of a Shield-1 treated sample showing two daughters enclosed by the double layer pellicle with limited anterior organelles, such as micronemes (M) and rhoptries (R). Note the large residual body (RB), limited by the plasmalemma, containing remnants of parasite organelles consisting of mitochondrion (Mi) and dense granules (DG). N, nucleus; G, Golgi body. Bar is 1 µm. (<b>C</b>) Irregularly shaped parasite showing abnormally located daughter inner membrane complexes (IMC), multiple nuclei (N), abnormal pre-rhoptries (R) and convoluted mitochondrion (Mi). Bar is 1 µm. (<b>D</b>) Apical end of a daughter exhibiting the nucleus (N), dilated Golgi body (G), rare micronemes (M) and abnormal rhopties (R) which lack ducts. Bar is 100 nm. (<b>E</b>) Detail of the perinuclear region showing an expanded Golgi body with a few vesicles with electron dense contents around the periphery (arrowheads). Mi, mitochondrion. Bar is 100 nm.