Non-invasive in situ cancer cells do not express decorin.

<p>Analyses were performed on the whole study population and representative images are shown. Asterisks indicate areas populated solely by bladder cancer cells. Panels A, D, G are representative images of serial sections of the same tissue sample representing in situ human bladder cancer. Arrows point to the borders of in situ carcinoma and asterisks indicate areas populated by bladder cancer cells only. A. HE staining. D. ISH for decorin. Positive DIG reaction in ISH indicating the localization decorin mRNA can be seen in purple. G. IHC for decorin. Brown color indicates decorin positive nonmalignant stromal cell areas. Parts of normal (B, E, H) and malignant (C, F, I) bladder tissue areas (asterisks) are shown magnified beneath. Note that in situ human bladder carcinoma cells are completely devoid of both decorin mRNA and decorin immunoreactivity and that decorin expression resides solely in the areas of original, non-malignant stromal tissue. In figures A, D, and G, scale bar 50 µm, and in B, C, E, F, H, and I, scale bar 20 µm.