Effect of the irradiation of FHN, XP-C and CS-B fibroblasts with low or high UVB doses during cell cycle progression:

<p>Cells were synchronized in G1, irradiated with different doses of UVB, and collected at the indicated times after irradiation. Fluorescence histograms were obtained for each condition after PI staining and flow cytometry. The proportion of cells in the G1, S and G2 phases of the cell cycle were analyzed and quantified for each histogram using ModFit LT. The results obtained were combined to generate the graphs presented. UVB doses used were: upper panels – non-irradiated controls; middle panels – low doses (750, 375 and 125 J/m² for FHN, XP-C and CS-B respectively); lower panels – high doses (2,000, 750 and 500 J/m² for FHN, XP-C and CS-B respectively).