Identification of splice limiting sequences at the 3’ ends of RON introns 10 and 11.

<p>(A) Time courses of splicing of transcripts of RON 10-11 and 11-12 with a branchpoint sequence and polypyrimidine tract from β-globin. The RON transcripts contain a consensus 5’ss at the 3’ end. (B) Time courses of splicing of β-globin transcripts in which sequences from the 3’ end of RON intron 10 replaced corresponding portions of the 3’ end of the β-globin intron. The sequence of the 3’ end of RON intron 10 is marked to show the 5’-most extremity and the lengths of the RON sequences used. The transcripts are labelled with numbers showing the number of nt of RON included and the number of nt of β-globin replaced (RON/β-globin). (C) Reactions as in (B) but with transcripts containing portions of RON intron 11, as shown.